NotyParticle Freemium

NotyParticle: Particle and Cell Image Analysis

Segment particles or cells, review masks and accepted objects, measure size and shape, and export calibrated particle-analysis results in NotyLab

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Features

Features & Customization

Reviewable segmentation

Use threshold-based OpenCV analysis, watershed assistance and available advanced paths while keeping the mask visible for review.

Particle size and shape metrics

Measure object area, diameter, circularity, aspect ratio, Feret dimensions and other morphology descriptors.

Calibrated data and reports

Use physical units when calibration is available and export particle data or supported PDF/HTML reports.

Turn a scientific image into reviewable particle measurements

NotyParticle is particle and cell image analysis software for segmenting objects, reviewing their boundaries and calculating size and shape metrics. It is designed around a review step: the segmentation is a starting point, and accepted objects should be checked against the source image before the measurements are used.

Choose a segmentation approach that matches the image

The browser OpenCV workflow supports threshold-based segmentation including Otsu, adaptive and manual threshold settings, plus preprocessing such as blur, opening and closing. Watershed can help split some touching objects. A Cellpose route is also represented in the workflow, but it depends on Local Engine and deployment availability and should not be assumed to be active everywhere.

Read the full guide: workflow, export checks and limitations

Review the mask before accepting measurements

Use the segmentation overlay, ROI controls and accepted/rejected object review to check whether the mask follows real object boundaries. Include or exclude regions when the full image is not representative, and filter obvious artifacts before generating final statistics.

Measure size and shape at object level

Available particle metrics include area, perimeter, equivalent diameter, circularity, aspect ratio, solidity, extent, major/minor axes, orientation, Feret dimensions, eccentricity and related shape descriptors. When a valid calibration is attached, supported size metrics can be reported in physical units; otherwise the measurements remain in pixels.

Inspect distributions, not only one average

Review summary statistics and size-distribution views together with the object table and quality information. A mean diameter can hide a mixed population, poor segmentation or a long tail, so the distribution and rejected objects are part of the scientific review.

A short workflow

  1. Load the image and attach a calibration if physical units are required.
  2. Choose OpenCV thresholding or another available segmentation path and tune it against visible object boundaries.
  3. Set the ROI and review the mask, especially merged, border-touching or obviously spurious objects.
  4. Accept the objects you want to analyze, inspect distributions and shape metrics, then export the required data or report.

Output you can keep

Export particle-level data as CSV and generate supported PDF or HTML reports. Keep the source image, calibration and segmentation settings with the result so a reviewer can understand how the object measurements were produced.

Segmentation limits to check

  • Otsu and other threshold methods work best when object and background intensity are sufficiently separable.
  • Touching or overlapping particles are not guaranteed to be separated correctly; watershed can assist but merged boundaries still need visual review.
  • Cellpose availability depends on the Local Engine and current deployment; do not base a required workflow on it without checking access first.
  • Calibration, image resolution and segmentation quality directly affect physical size statistics.

Continue your work

Use cases

Use Cases

Who NotyParticle is for

  • Materials researchers measuring particles, pores or other segmented objects in microscopy images
  • Students learning how threshold and segmentation choices affect particle statistics
  • Researchers who need object-level size and shape data with an inspectable mask and acceptance step

Open NotyParticle in NotyLab

Open the Tool directly. You can switch between Tool and Workflow mode from the toolbar without leaving the module

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