Analyze Phase Percentage

Identify phase regions, calculate area percentages, and review the overlay before export.

NotyLibrary -> NotyCheck -> NotyPhase

Intermediate 4-8 min
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Best for I want to estimate the percentage of visible phases in a microscopy image.
Input Microscopy Image, SEM, Optical Microscopy, PNG, JPG, TIFF
Output Reviewed phase overlay and percentage result
Estimated time 4-8 min

Before you start

Make sure your image has at least one reliable scale reference. This can be an existing scale bar, a saved calibration, or a known pixel-to-unit ratio. If the image is already calibrated, you can skip calibration setup and continue to Edit or Scale & Export.

  • Microscopy image is ready
  • Calibration reference is available
  • Output format is selected
  • Crop or image cleanup is only used when needed

See the workflow in 30 seconds

This short video shows the full path: import the image, select or create calibration, crop or improve the image, add the scale bar, and export the final result.

30-second workflow preview

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What you will learn

  • Where to start
  • When calibration is required
  • When to use Edit
  • How to export the final image

Workflow Steps

1
NotyLibrary

Organize the phase-analysis file set

Bring the image, notes, and follow-up exports into one project context.

2
NotyCheck

Review image quality before segmentation

Confirm contrast and visual separation are good enough for phase work.

3
NotyPhase

Identify phase regions

Apply the phase analysis step and build the visual overlay.

4
NotyPhase

Calculate area percentages

Summarize the detected phase distribution in clear scientific terms.

5
NotyCheck

Review overlay readability and export

Check that the overlay remains interpretable in the final output.

Common mistakes this workflow helps prevent

Low-contrast regions that are hard to separate

Overlays that hide important features

Percentages reported without a review snapshot

Expected output

Before

Raw microscopy image with old scale context, unnecessary borders, or no final scale bar.

After

Clean microscopy image with a calibrated, readable scale bar placed in a safe corner.

Export-ready for

Reports, lab notes, scientific slides, manuscripts, documentation, and tutorials.

Frequently asked questions

Should I review the image before phase analysis?

Yes. Better contrast and cleaner inputs make the phase review more interpretable.

Can the overlay be exported for reports?

Yes. This workflow is designed to keep the review overlay and the result together for export.

Ready to calibrate and export your image?

Start with your microscopy image, select calibration, edit only if needed, then add a clean scale bar and export.

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